Titanium dioxide modified target plates were developed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to enhance intact bacterial analysis. The plate is designed to photocatalytically destroy the bacterial envelope structure and increase the ionization efficiency of intracellular components, thereby promoting a measurable mass range and achievable detection sensitivity. Thus, a method for rapid detection of antimicrobial resistance-associated proteins has been established by mass spectrometric fingerprinting of intact bacteria, which confers resistance to microbial drugs to the bacteria without any sample pretreatment. In this way, changes in the expression level of the resistance protein in the bacteria can be rapidly measured from the relative peak intensities. This method of detecting resistance proteins directly from intact bacteria by mass spectrometry can be used to rapidly distinguish between antimicrobial resistant bacteria and their non-resistant counterparts while performing species identification. In addition, it can be used as a quick and convenient way to initially identify potential resistance mechanisms.